Dynamic imaging and quantification of subcellular motion with eigen‐decomposition optical coherence tomography‐based variance analysis

Subcellular imaging of epithelium with time-lapse optical coherence tomography.

We present the first experimental result of direct delineation of the nuclei of living rat bladder epithelium with ultrahigh-resolution optical coherence tomography (uOCT). We demonstrate that the cellular details embedded in the speckle noise in a uOCT image can be uncovered by time-lapse frame averaging that takes advantage of the micromotion in living biological tissue. The uOCT measurement ...

Real-time bulk-motion-correction free Doppler variance optical coherence tomography for choroidal capillary vasculature imaging

In this paper, we analyze the retinal and choroidal blood vasculature in the posterior segment of the human eye with optimized color Doppler and Doppler variance optical coherence tomography. Depth-resolved structure, color Doppler and Doppler variance images are compared. Blood vessels down to the capillary level were detected and visualized with the optimized optical coherence color Doppler a...

Visible Spectrum Optical Coherence Microscopy for Live Subcellular Imaging

Speckle variance optical coherence tomography for imaging microcirculation

Non-invasive flow detection and in-vivo measurement are one of the most requested clinical applications for medical imaging modalities to investigate vascular pathologies and responsiveness to drug therapy. Implementing speckle variance calculation & visualization in a market-proven optical coherence tomography (OCT) system we demonstrate an angiography imaging algorithm that provides angle ind...

Motion correction for phase-resolved dynamic optical coherence tomography imaging of rodent cerebral cortex

Cardiac and respiratory motions in animals are the primary source of image quality degradation in dynamic imaging studies, especially when using phase-resolved imaging modalities such as spectral-domain optical coherence tomography (SD-OCT), whose phase signal is very sensitive to movements of the sample. This study demonstrates a method with which to compensate for motion artifacts in dynamic ...